Imagej trackmate

Most of it's functions can be scripted in various languages and the Fiji distribution can also run in headless mode. g. trackscheme. and has an automatic update function, bundles a lot of plugins and offers comprehensive documentation. Doing so, I could not detect a single spot in the example file provided in fiji. 9KImageJ - New plugin released: TrackMate - a plugin for imagej. AND INFORMATION MINING CHAIR FOR BIOINFORMATICS ImageJ Forum; TrackMate documentation; MOSAIC suite for ImageJ/Fiji documentation; ilastik ilastik download; Pixel classification documentation; Icy How can I save a set of Tracks generated on Learn more about tracking, trackmate, tif, imagej Bio-Formats Plugins: a collection of plugins for ImageJ, including the Bio-Formats Importer, Bio-Formats Exporter, Bio-Formats Macro Extensions, Data Browser and Stack Slicer. So it is a good idea to pre-process, crop, edit metadata and massage the target image first in …TrackMate FAQ This list of frequently asked questions is compiled from several questions we have seen several times on the ImageJ forum. TrackMateによるTracking. 2005/06/15: New features: 2D TrackMate spot filter on 'Maximum intensity' (28. General Description. In this review, fiji. In order to capture the intensity at time points before the drople README. ui. The TrackMate_ class runs on the currently active time-lapse image (2D or 3D) and both identifies and tracks bright spots over time. fabrice. TrackMate is your buddy for your everyday tracking. Jan Eglinger ( imagejan on github and the ImageJ forum) Friedrich Miescher Institute for Biomedical Research (FMI) Imagej User Guide 2009-07-10 - FIJI presentation from ELMI2009 ImageJ Tutorial Manual editing of tracks using TrackMate · Manual tracking with TrackMate · Miji · Moving Least. Berthold Abstract The open analytics platform KNIME is a modular environment that en- For each strain, 598 cell tracks were evaluated (TrackMate plugin for ImageJ). The internet speed test trusted by millions. net/Scripting_TrackMate#Export_spot. Imagej Manual Segmentation TrakEM2 manual segmentation modes. TrackMate (http://imagej. 発表論文 Publication (Google Schalor) 研究費 Funding (Researchmap) Recruit and contact. MOSAIC suite is an ImageJ/Fiji plugin that can perform tracking and export the traces as an ImageJ table. Required input: A 2D or 3D Author: Fabrice Cordelières, Institut Curie, Orsay (France). We’re going to be using TrackMate which comes This is available from their website as an ImageJ 2 thoughts on “ Staying On Track Update: I put the link to the jar and code of the track analysis module on the TrackMate page for extensions: ImageJ TrackMate. The 'ImageJ Plugins' project is a source of custom plugins for the Image/J software. md TrackMate. In this video, I use LoG detection and filter for ~30 high quality tracked lipid droplets. 2C_edge I have managed to do the image sequence and run the TrackMate in MATLAB using the ImageJ-MATLAB as shown in two crucial steps below. 1-01 Loading Nexus UI Trackmate LoG detector apprently runs endlessly without providing any output (tested on different time-lapses and for different threshold values). Dear all, I'm trying to do cell tracking using the plugin "Trackmate" in Fiji. For example, in the case of Trackmate plugin for ImageJ, [gembloong_ads1] [gembloong_ads2] image gallery module diagram sensors free full text untitled document [ ] "imagej ubuntu meta image plugin" "imagej 3d "imagej ubuntu meta image plugin" "imagej 3d image gallery module diagram sinusitis free full text iplaminator an imagej plugin for automated binning and trackmate wiring diagram 24 wiring MosaicSuite for ImageJ and Fiji. It offers a May 10, 2012 Dear friends and colleagues, We just released a new Fiji plugin dedicated to automated & manual spot tracking, TrackMate. van Wezel Imagej Plugins Manual Tracking 1. Hello everyone, I am attempting to track cell migration using the TrackMate plugin in Fiji and I have a couple questions. How to free up memory when analyzing images Learn more about java, image processing, imagej, fiji, trackmate MATLAB ImageJ and Matlab Possible TrackMate: http://fiji. 12. sc/TrackMate Form: ImageJ/Fiji plugin METHOD 13 Authors: Joost Willemse, Katherine Celler, Gilles P. Purpose. Test definition is - a means of testing: such as. Webinar Image Processing in KNIME Martin Horn and Christian Dietz ImageJ Trackmate VTK ImageJ3D Viewer OMERO SUISE Trackmate Allows tracking of objects in KNIME This tutorial workflow shows some simple ImageJ functionality in KNIME. fr Software: http://fiji. Make Fiji Package · Mamed · Manual editing of tracks using TrackMate · Manual Cell Profiler Cell Profiler Analyst • „Fiji compares to ImageJ as Ubuntu compares to Linux. nabble. Imagej User Guide 2009-07-10 - FIJI presentation from ELMI2009 ImageJ Tutorial Manual editing of tracks using TrackMate · Manual tracking with TrackMate · Miji · Moving Least. Particle tracking plugins file but since I’m a big fan of ImageJ I am interested in importing data from MTrackJ to Trackmate and so I must convert the Posts about Image Analysis written by Dave Mason. 1557. Using the program ImageJ with the plug-in TrackMate, I can take the experimental images and analyze the microtubule’s movements over a time period. HPLC - Isocratic vs I am currently writing a few Trackmate scripts to automate my Trackmate Scripting Output to CSV http://imagej. findmaxima. imagej trackmate TrackMate is a plugin within the Fiji ImageJ distribution TrackMate: an open and extensible platform for single-particle tracking2. problem with Trackmate. 1) Tracking mitochondrial movements using imageJ? stepwise protocol to track and calcuate the distance travelled using imageJ? give it a try with TrackMate. Posts about ImageJ written by Dave Mason. m2/repository/sc/fiji/TrackMate_/3. Dear friends and colleagues, We just released a new Fiji plugin dedicated to automated & manual spot tracking,I tried to get used to Trackmate in ImageJ (using Fiji) and followed the basic tutorial. Imagej Plugins Manual Tracking 1. MTrackJ is an ImageJ plugin to facilitate tracking of moving objects in image sequences and the measurement of track statistics. This tutorial workflow shows some simple ImageJ functionality in KNIME. Make Fiji Package · Mamed · Manual editing of tracks using TrackMate · Manual [gembloong_ads1] [gembloong_ads2] image gallery module diagram sensors free full text untitled document [ ] "imagej ubuntu meta image plugin" "imagej 3d "imagej ubuntu meta image plugin" "imagej 3d image gallery module diagram sinusitis free full text iplaminator an imagej plugin for automated binning and trackmate wiring diagram 24 wiring How to free up memory when analyzing images Learn more about java, image processing, imagej, fiji, trackmate MATLAB About r/ImageJ. This list of frequently asked questions is compiled from several questions we have seen several times on the ImageJ forum. Alternatively, copy MTrack2_. Undergraduate Volunteer University of California San Diego. m2/repository/net/imagej KNIME for Open-Source BioImage Analysis - A Tutorial Christian Dietz and Michael R. TrackMate relies on several different libraries and plugins for data manipulation, analysis and visualization. net/TrackMate) is one of the most extensive plugins for ImageJ, ImageJ Trackmate Moving Lipid Droplet Tracking - DoG Detector. A plugin in Fiji. ImageJ, Fiji Particle Analysis - ImageJ- Manual1. txt · Last Scientists Like it Fast performed by using ImageJ software and TrackMate-algorithm. Blend tools and data types seamlessly The freedom to continue using your favorite tools (as you like) while trying out new ones – all within the same environment Blending data from many sources and types on the platforms of your choice in a consistent visual interface free of propr Workflows and data are available in the KNIME EXAMPLES Server under 50_Applications/18_Churn_Prediction50_Applications/18_Churn_Prediction* Getting started with TrackMate is a basic tutorial that explains how TrackMate works on an easy image. So, bear with me. List of tests Test your Internet connection bandwidth to locations around the world with this interactive broadband speed test from Ookla. FindMaximaSpotDetector Quantitating the cell: turning images into numbers with ImageJ. Citation. In this review, The TrackMate (41) ImageJ plugin was used for advanced cluster dynamic analysis. I know that in ImageJ/Fiji I can use the Plot Profile function to select a line profile of an image. 2C_edge Fiji は ImageJ とほぼ同じですが、便利な Plugin Plugins > Tracking > TrackMate で TrackMate を立ち上げると、下の window Fiji is easy to install. In the end it can give you nice movies like [TrackMate Email: tinevez@pasteur. - 2D tracking and 3D tracking using - Trackmate and results interpretation using Mamut - More freedom in analyzing images using ImageJ and Matlab. net/TrackMate) for some velocity analysis, but it seems to only look for perfect circles. Trackmatestatisticsforuilclass1a. History: 2004/06/25: First version. top of page Create a Time-Series Stack with ImageJ. For example, in the case of Trackmate plugin for ImageJ, A Java package for bi-directional communication and data exchange from Matlab to ImageJ/Fiji ImageJ has several tracking plugins, a good one being TrackMate. -Analyze chemotactic behavior of cells using ImageJ TrackMate and MATLAB. As part of my job, I find myself writing lots of bits of code for people. If you use it successfully for your research please be so kind Mar 29, 2016Feb 15, 2017 We present TrackMate, an open source Fiji plugin for the automated, semi-automated, and manual tracking of single-particles. Nexus Repository Manager OSS 2. TrackMate honors ImageJ thread configuration. The following instructions explain how to import a wide variety of compressed videos into ImageJ run on windows. Send comments to wsr nih. 2009-07-10 - FIJI presentation from ELMI2009 ImageJ Tutorial M. MTrackJ is launched by selecting it from the Plugins menu of ImageJ. It was used to work but since two days, I always have this error message when launching Using Trackmate Manual Annotation to Track Moving Lipid Droplets and Measure Intensity Over Time I'll try to make a more detailed tutorial in the future! ImageJ Trackmate Moving Lipid Droplet Tracking - DoG Detector - Duration: 4:41. Please note that TrackMate is available through Fiji, and is based on a publication. 1 Examples, 1. u-psud. 5. DefaultOpFinderService (implements net. Proceedings of the National Academy of Sciences Jun 2017, 114 (24) 6340-6345; . If you have not already done so, create a Week 3 directory or folder on your hard drive for this week's work. fr. KNIME Image Processing What’s new? Christian Dietz, ImageJ 3D Viewer Integration 4 Tracking with TrackMate 6 This category is for announcements: A new plugin to import CSV files into TrackMate (2) VOTE NOW for the New ImageJ Logo! (9) First of all, I'm a total "signal processing" newbie. • TrackMate • ImageJ1-Macro Why KNIME & ImageJ? •Joined Efforts of two Leading Communities •Big Image Processing and Analysis •Exploration and Prototyping It also comes bundled up in ImageJ/FIJI so it fits neatly into my workflow with the minimum of fuss. In the end it can give you nice movies like [TrackMate ParticleTracker 2D and 3D An ImageJ Plugin for multiple particle detection and tracking from digital videos Written by Guy Levy and maintained by Janick Cardinale at I have recently downloaded Icy and I am currently learning to use it. sc/TrackMate MIJ is an Open-source Java class facilitating interoperability between Matlab and ImageJ/Fiji. 4:41. Like ImageJ itself, Fiji is an open source project hosted in Git version control repositories, with access to the source code of all internals, libraries and plugins, easing the development and scripting of plugins. plugin. Publication. cordelieres at curie. ELICEIRI* Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, Wisconsin SUMMARY Technology in Bioinformatics course timetable Show (e. jar Note that ImageJ also ships a unified Script Interpreter plugin, accessible from Plugins Scripting Script Interpreter. Xfinity Speed Test tests your Internet connection speed. 04 KB, image/png) 2775ecd7 (UPDATEABLE) 20150914131926 jars/imagej-plugins-commands-0. New HTML5 speed test, no Flash Check the speed, quality and performance of your Internet connection with the AT&T Internet speed test. Features, Fiji, ImageJ, Linkage, Mosaic Toolsuite, MPI-CBG, Time, Tracking, TrackMate on July 20, But more importantly, I would like to highlight several contributed TrackMate modules since the last release. net/TrackMate) is one of the most extensive plugins for ImageJ, Posted under Fiji, ImageJ, Science, Software on December 3rd, TrackMate for the automatic tracking and MTrackJ for the manual and track editing/visualization. HINER, AND KEVIN W. class and MTrack2_$particle. ImageJ is a freely Motion detection with ROI (self. ConvolveFFTRAI at fiji. Overlaying fluorscence and DIC Open the images in ImageJ Adjust the contrast if neceesary: Image/Adjust/BrightnessConstrast Change or check all Images are in 8-bit format. MTrackJ: An ImageJ Plugin for Motion Tracking and Analysis. Schindelin e. net. Color RGB Stack Splitter RGB Stack Merge HSB Stack Splitter RGB We present a software module MIJ[1] that allows to combine the powerful numerical computation of Matlab[2] and the image-analysis capabilities of ImageJ[3]. nih. When loading a TrackMate file, it tries first to retrieve and open this file in ImageJ. 2. Fiji, ImageJ のメモ MultiTracker uses ImageJ's particle analyzer to track the movement of multiple objects through a binary or thresholded stack. . Image Segmentation, Single-Molecule Tracking, and Data Analysis. Bio-Formats Plugins: a collection of plugins for ImageJ, including the Bio-Formats Importer, Bio-Formats Exporter, Bio-Formats Macro Extensions, Data Browser and Stack Slicer. a guest Apr 21st, at net. ImageJ alone can only import uncompressed videos. Online tests and testing for certification, practice tests, test making tools, medical testing and more. Enhances KNIME by providing algorithms and data structures to process and analyse images. exc. It was used to work but since two days, I always have this error message when launching TrackMate Saving Bug!. How to use test in a sentence. Fiji は ImageJ とほぼ同じですが、便利な Plugin Plugins > Tracking > TrackMate で TrackMate を立ち上げると、下の window This page has been visited times. I particularly like the spot detection and tracking plugins, but I am also using TrackMate from ImageJ. Particle Analysis . 1. Posted under Fiji, ImageJ, Science, Software on December 3rd, TrackMate for the automatic tracking and MTrackJ for the manual and track editing/visualization. Tinevez, J. One of the most prominent is the open‐source image package ImageJ. detection. We expect experience in handling image data and familiarity with imageJ/FIJI; Analyst & Developer of TrackMate @ Pasteur Institute – Paris, France; Download ImageJ Plugins for free. ESTIMATION OF PROTEIN AGGREGATE DENSITY USING Tracks determined using Trackmate variable threshold plugin for ImageJ/FIJI • TrackMate: Follow objects over time • Tess4J: Optical Character Recognition in KNIME knime. Explore the latest articles, projects, and questions and answers in ImageJ, and find ImageJ experts. Disclaimer nih. Hello everyone, Recently I've been trying to extend TrackMate so that I can pull start and stop X and Y positions of the tracks in the track problem with Trackmate. You should start here. x6. ParticleTracker The ParticleTracker is an ImageJ/Fiji Plugin for multiple particle detection and tracking from digital videos Sample Data and Tutorial TrackMate simplifies the export and exchange of data and results with other tracking tools and/or analysis applications. But it is currently beta quality, and the Python language does not work properly due to bugs. com/New-plugin-released-TrackMate-a-pluginNew plugin released: TrackMate - a plugin for easy tracking. ImageJ) submitted 3 years ago by gabio11. References. Download the data. TrackMate plugin for Fiji. TrackMate DownsampledLogSegmenter. TrackMate. TrackMate Use the menu command Plugins Fiji, ImageJ. swing. gov. Click here for more information! TrackMate Use the menu command Plugins Tracking TrackMate. I've had good results using the [trackmate plugin Image analysis for biologists (e. What is MosaicSuite? Several of the image-processing algorithms developed at the MOSAIC Group for fluorescence microscopy are available as plugins for the popular free image processing software ImageJ2 or Fiji. Use our free bandwidth test to check your speed and get the most from your ISP. This will open the MTrackJ dialog shown below, which is then associated with the currently active image and visually attached to its window. The most simple way to get started is to use the Script Editor, which takes care of the difficult & boring part for you (such as path). The object number and coordinates may The ImageJ ecosystem: An open platform for biomedical image analysis. The ImageJ ecosystem: An open platform for biomedical image analysis. TrackMate , a The TrackMate (41) ImageJ plugin was used for advanced cluster dynamic analysis. Good Intensity even at Bright Backgrounds Figure 1 (bottom) shows a bead which Checking URL: file:/D:/Users/Jean /Users/Jean-Yves/. Prodanov, J. TrackMate Use the menu command Plugins I tried to import TrackMate xml track file into We have used imagej and Fiji for quite a long time and we has also noticed that Icy has an excellent 3D [gembloong_ads1] [gembloong_ads2] image gallery module diagram sensors free full text untitled document [ ] "imagej ubuntu meta image plugin" "imagej 3d "imagej ubuntu meta image plugin" "imagej 3d image gallery module diagram sinusitis free full text iplaminator an imagej plugin for automated binning and trackmate wiring diagram 24 wiring Imagej Manual Tracking Calibration Tutorials focused on usage of ImageJ. OpFinderService) org. za keyword after analyzing the system lists the list of keywords related and the list of Trackmate imagej. 2 Single Particle Tracking, 1. It will be updated as questions appear there. Uploaded by Cookbook Tutorials 09-Oct-14 4:30 PM . trackmate. imagej. License: GPL: Used By: 9 artifacts: Loci (1) ImageJ Releases (43) TrackMate simplifies the export and exchange of data and results with other tracking tools and/or analysis applications. mainpages/links2011. gov/ij/). October 2017 – December 2017 I am new to using imageJ and creating my own codes, anyways, I am trying to create a loop that runs all the roi's at once, but I am having trouble doing that. So far this is the code I have: inpu Re: [fiji-devel] Reproducible pom - TrackMate - TrackMate-examples we cannot upload new versions of any of these components until the ImgLib2 release itself Posts about ImageJ written by Dave Mason. If you use it successfully for your research please be so kind to cite our work: This tutorial is the starting point for TrackMate users. It …Mar 30, 2016 · ImageJ Trackmate Moving Lipid Droplet Tracking - DoG Detector Conaanaa, also known as Suppy. I tried to get used to Trackmate in ImageJ (using Fiji) and followed the basic tutorial. (30 plugins) Software packages such as ImageJ, MATLAB, CellProfiler or ICY are often used to analyze bioimages. File -> Open Samples -> Tracks for Trackmate 今回は、ImageJという画像解析ソフトを使った画像位置合わせについてご紹介します。 I'm using [Trackmate](http://imagej. Image/J is a public domain image processing and analysis program developed in Java (http://rsb. ImageJ, Matlab, we will cover time series processing and cell tracking using TrackMate. Ca2D imaging in live GFP-RyR2 expressing cells via a TIRF microscope Trackmatic. ContextCommand EMBL Heidelberg Events 2017 Bioimage Data Analysis Bioimage ImageJ Basics - ImageJ Trackmate and results interpretation using Mamut ImageJ is an image analysis program extensively used in the biological sciences and ImageJ for the next generation of scientific image data. TrackMate also should output average The following instructions explain how to import a wide variety of compressed videos into ImageJ run on windows. command. 2. [gembloong_ads1] [gembloong_ads2] image gallery module diagram sensors free full text untitled document [ ] "imagej ubuntu meta image plugin" "imagej 3d "imagej ubuntu meta image plugin" "imagej 3d image gallery module diagram sinusitis free full text iplaminator an imagej plugin for automated binning and trackmate wiring diagram 24 wiring REVIEW ARTICLE Molecular Reproduction & Development (2015) The ImageJ Ecosystem: An Open Platform for Biomedical Image Analysis JOHANNES SCHINDELIN, CURTIS T. Imagej Manual Tracking Calibration Tutorials focused on usage of ImageJ. Sage, D. imagej trackmateTest(s) or TEST may refer to: Test (assessment), an assessment intended to measure the respondents' knowledge or other abilities. I am currently writing a few Trackmate scripts to automate my Trackmate Scripting Output to CSV http://imagej. visualization. It explains how it works by walking you through a simple case, using an easy image. Conaanaa, also known as Suppy 6,997 views. ImageJ, Fiji Software packages such as ImageJ, MATLAB, CellProfiler or ICY are often used to analyze bioimages. ops. RUEDEN, MARK C. 2C_edge WHY TRACKMATE? Since we started Thanks trackmategps for my peace of mind that my property is where I expect it to be when I can't be around to watch it myself! Fiji is easy to install. Close everything such as Pandora, Netflix, Hulu, Spotify, all browser windows and tabs (except the one you're using for the test) and any other programs that From Old French test (“an earthen vessel, especially a pot in which metals were tried”), from Latin testum (“the lid of an earthen vessel, an earthen vessel, Mar 10, 2018 Please note that TrackMate is available through Fiji, and is based on a publication. • TrackMate • …. License: GPL: Used By: 9 artifacts: Loci (1) ImageJ Releases (43) ここでは動画解析の事例として上に示した動画,特に油滴の追跡を行います. まず以上の動画だけでは大きすぎるのでImageJに読みこませた後,不必要な場所を消去しましょう.このような不要な部分の消去は記憶容量の Particle Analysis - ImageJ- Manual1. Launching and Quitting. It also comes bundled up in ImageJ/FIJI so it fits neatly into my workflow with the minimum of fuss. 3 TrackMate ImageJ plugin with a GUI, Trackmate LoG detector apprently runs endlessly without providing any output (tested on different time-lapses and for different threshold values). Quantitating the cell: turning images into numbers with ImageJ. net/Scripting_TrackMate#Export_spot. Loading Unsubscribe from Conaanaa, also known as …Author: Conaanaa, also known as SuppyViews: 6. png. How to manually segment cells using the 3 segmentation. The use of ImageJ can allow a user to colour their image, without any additional software which can be a great benefit especially if the user has become comfortable with ImageJ. co. scijava. convolve. But more importantly, I would like to highlight several contributed TrackMate modules since the last release. net/TrackMate) is one of the most extensive plugins for ImageJ, UCSD Confocal Microscopy Plugins MBF ImageJ for Microscopy Collection by Tony Collins. The KNIME Image Processing Extension uses and integrates state-of-the-art libraries such as ImageJ1 and ImageJ2, SCIFIO, OMERO, ClearVolume, ImgLib2, CellProfiler, TrackMate and others. 3 TrackMate ImageJ plugin with a GUI, Update: I put the link to the jar and code of the track analysis module on the TrackMate page for extensions: ImageJ TrackMate. Search results for: Imagej analyze particles summary writing. TrackScheme: the tool for the visualization, editing and analysis of tracks. 1-SNAPSHOT/TrackMate_-3 /Users/Jean-Yves/. info. java to your ImageJ plugins folder and use the TrackMateによるTracking. 1-01 Loading Nexus UI Download ImageJ Plugins for free. Daniel Sage: MIJ: Making Interoperability Between ImageJ and Matlab Possible D. Dear friends and colleagues, We just released a new Fiji plugin dedicated to automated & manual spot tracking, Using TrackMate to track single cell migration. Spot-On↔TrackMate connector. This can be a pitfall when distributing a complex plugin, but this is where the Fiji magic comes into play. ImageJ , Matlab, Micro we will cover time series processing and cell tracking using TrackMate and advanced image “Fiji is Just ImageJ”,functioning also as a platform for open-source development ImageJ TrackMate. class to the plugins folder and restart ImageJ. Time series processing and cell tracking using TrackMate: Richard Butler, Jeremy Pike: (2D/3D) ImageJ interaction Plug-in installation Image annotation, simple How can I save a set of Tracks generated on Learn more about tracking, trackmate, tif, imagej I personally have used ImageJ in headless mode scripted with its own macro language because it is relatively easy to learn. I am currently writing a few Trackmate scripts to automate my Trackmate Scripting Output to CSV http://imagej. -Y. [gembloong_ads1] [gembloong_ads2] image gallery module diagram sensors free full text untitled document [ ] "imagej ubuntu meta image plugin" "imagej 3d "imagej ubuntu meta image plugin" "imagej 3d image gallery module diagram sinusitis free full text iplaminator an imagej plugin for automated binning and trackmate wiring diagram 24 wiring Get expert answers to your questions in ImageJ, Particle Tracking, Trackmate and ImageJ/Fiji and more on ResearchGate, the professional network for. Ca2D imaging in live GFP-RyR2 expressing cells via a TIRF microscope But more importantly, I would like to highlight several contributed TrackMate modules since the last release. New plugin released: TrackMate - a plugin for easy tracking. . Disclaimer I tried to get used to Trackmate in ImageJ (using Fiji) and followed the basic tutorial. Copy MTrack2_. Dear Fiji/TrackMate Developers, TrackMate is unable to save files under Win7! After finishing a detection, the "Save" button just locks the TrackMate window. TrackMate_ can also be used without the GUI, using a scripting language that allows making calls to Java